Journal: Biological Procedures Online
Article Title: Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimulons and regulons in bacteria with sequenced or partially sequenced genomes
doi: 10.1251/bpo110
Figure Lengend Snippet: Samples to be compared are grown (A), after harvesting the proteins are solubilized (B). The crude protein mixture is then applied to a first dimension tube gel that separate the proteins based on their isoelectric points (C). After this step, the gel is applied to a second dimension SDS-PAGE gel where proteins are denatured and separated on the basis of size (D). Gels are fixed and stained by coomassie or silver staining (E). The resulting protein spots are recorded and quantified (F). The spots of interest are then excised (G) and subjected to mass spectrometric analysis.
Article Snippet: IP and MW were determined by using a 2D SDS-PAGE standard (Bio-Rad, Hercules, CA).
Techniques: SDS Page, Staining, Silver Staining